Technologies2A peptide recoding of viral segments
viral engineering
Recorded terms: 2A peptide polycistronic design; 2A ribosome recoding
2019 · Cold Spring Harbor Perspectives in Medicine · lab-led
Frames influenza A virus as a compact genetic circuit whose modules can be disrupted, tagged or replaced, and organizes two decades of virus engineering into a design vocabulary of tracking, override, positioning and silencing modules that turns virus construction into a method of inquiry.
2013 · Cell Reports · lab-led
The inefficient 5 prime splice site of influenza A virus segment 8 functions as a timing device, causing the nuclear export protein to accumulate slowly as a minor product of abundant NS1 transcription, with both raising and lowering that rate attenuating the virus through mistimed ribonucleoprotein export.