Technologies
Controlled terms connect methods and biological systems across the research program.
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- Quantitative RT-PCR
- Bulk RNA sequencing
- Plaque assay and TCID50 titration
- Reverse genetics and virus rescue
- Immunofluorescence and confocal microscopy
- Small interfering RNA and short hairpin knockdown
- Small RNA deep sequencing
- Flow cytometry
- Small RNA northern blot
- MicroRNA target site insertion into viral genomes
- Gene ontology and gene set enrichment analysis
- Luciferase promoter reporter assay
- Immunoblotting
- Site-directed and alanine scanning mutagenesis
- Route-controlled in vivo infection and delivery
- Histopathology
- Northern blot
- Deep sequencing of viral populations
- Electrophoretic mobility shift assay
- ELISA
- Immunohistochemistry
- Multicycle growth curves
- Multiplexed cytokine measurement
- Small molecule inhibitor profiling
- Barcoded virus libraries
- In vitro reconstitution with purified components
- Adenoviral vector delivery
- Animal transmission models
- Coimmunoprecipitation
- CRISPR-Cas9 knockout cell lines
- In vitro kinase assay
- Lentiviral transduction
- Mass spectrometry proteomics
- Phylogenetic and coalescent analysis
- Post-transcriptional silencing reporter assay
- Pseudotyped entry reporters
- RNA in situ hybridisation
- Single-cell RNA sequencing
- Virus-encoded artificial microRNAs
- Amplicon sequencing
- Argonaute immunoprecipitation
- ATAC sequencing
- Cell and nuclei sorting
- Cell viability and cell death assays
- Chromatin immunoprecipitation and CUT&RUN
- Conditional and inducible gene knockout
- Dimensionality reduction and clustering
- Genome-wide CRISPR-Cas9 knockout screening
- In vivo RNAi screening through viral fitness
- Knockout mouse infection
- Locked nucleic acid antisense inhibition
- Minigenome, minireplicon and replicon reporters
- Primer extension
- Radiolabelling and translation measurement
- Serial passage and selection
- Short-read sequencing platforms
- 2A peptide recoding of viral segments
- Antigen-specific B cell detection
- Bacterial artificial chromosome recombineering
- Behavioural and sensory testing
- Cell type deconvolution of bulk transcriptomes
- Comparative genomics and conservation analysis
- Cre-LoxP lineage tracing of infected cells
- Differential expression analysis
- Directed differentiation of human pluripotent stem cells
- Doxycycline-inducible lentiviral expression
- Dye dilution cell division tracking
- Engineered transcription factor constructs
- Expression microarray
- Phosphoproteomics
- Phosphospecific immunoblotting and phosphatase treatment
- Plaque reduction neutralisation test
- Population diversity statistics and simulation
- Promoter motif discovery and enrichment
- Replication-incompetent influenza vector
- RNA immunoprecipitation
- Stem-loop quantitative RT-PCR for small RNAs
- Subcellular fractionation
- Synthetic and chemically modified RNA mimetics
- Targeted in-solution hybridisation capture
- Transcription factor activity and motif accessibility inference
- 5 prime RACE
- Adeno-associated virus vectors
- Adoptive cell transfer
- Agroinfiltration of plant tissue
- Air-liquid interface culture
- Amplification on permissive cells to detect low-level infectious virus
- Ancient DNA extraction
- Bio-layer interferometry
- cDNA complementation of knockouts
- Cellular cholesterol quantification
- Cellular reprogramming to pluripotency
- Chimeric minigene splicing reporters
- Combinatorial peptide substrate specificity profiling
- Cross-dataset meta-analysis
- De novo transcriptome assembly
- Deamination damage authentication
- Deep mutational scanning
- Electron microscopy
- Epithelial barrier permeability measurement
- Expression screening of codon-optimised heterologous proteins
- Fluorescent and luciferase reporter viruses
- Fluorescent fusion protein localisation
- Genetic panels lacking small RNA machinery
- Head-to-head competition assay
- Hemagglutination inhibition assay
- High-content microscopy
- Host genetic ancestry analysis
- In situ Hi-C and compartment analysis
- Interferon bioassay
- Kidney capsule xenotransplantation
- Kinase activity inference
- Lectin staining of surface glycans
- MHC class I tetramer staining
- MHC epitope prediction
- Morpholino knockdown
- mSwAP-In iterative genome writing
- Neutral red light-sensitive virus labeling
- Noncanonical splice junction read analysis
- NS1-deleted influenza A virus
- Organ-on-a-chip microfluidics
- Organoid culture
- Peptide restimulation of T cells
- Pharmacokinetic analysis
- Pharmacological immunosuppression
- Phos-tag gel electrophoresis
- Radiocarbon dating and strontium isotope analysis
- RNA affinity tagging of viral genomes
- SELEX selection of bound RNA
- Serum transfer with ultraviolet inactivation
- Shotgun metagenomic sequencing
- Single-cell CRISPR screening with ECCITE-seq
- Single-molecule FRET
- Size-exclusion chromatography
- Small molecule-assisted shutoff of viral proteins
- Split NS segment 8 design
- Suppressor-deficient virus mutants
- T cell hybridoma antigen presentation assay
- Targeted cell ablation
- Tetraploid blastocyst complementation
- Trans-complementation infection assay
- Transcription and translation inhibitor blocks
- Transposon insertional mutagenesis of viral genomes
- UV crosslinking immunoprecipitation sequencing
- VP55-mediated ablation of the cellular microRNA pool