TechnologiesEngineered transcription factor constructs
functional genomics and screening
Recorded terms: chimeric VPR transcriptional activators; constitutively active IRF7 truncation
2021 · Journal of Virology · lab-led
SARS-CoV-2 infection of human lung epithelial cells engages NF-κB at chromatin, transcriptional, protein and post-translational levels without engaging the type I interferon transcription factors, and loss of p65 or p50 abolishes viral replication in a manner rescued by reconstituting RelA transcriptional activity.
2019 · Proceedings of the National Academy of Sciences · lab-led
Forcing an interferon-stimulated gene program in human induced pluripotent stem cells with a constitutively active IRF7 produces lasting transcriptional change and impaired germ layer differentiation, supporting the proposal that the canonical type I interferon system and pluripotency are difficult to hold simultaneously.