tenOever LaboratoryVirology · Host defense · RNA biology
Technologies

Directed differentiation of human pluripotent stem cells

animal, organoid and tissue models

Recorded terms: directed differentiation of human pluripotent stem cells; directed trilineage differentiation; embryoid body cardiomyocyte differentiation; hPSC ScoreCard assay

2020 · Cell Stem Cell · collaborative

A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids

A panel of eight human pluripotent stem cell derivatives spanning all three germ layers, together with adult primary islets and liver organoids, identifies pancreatic alpha and beta cells, hepatocytes, cholangiocytes, cardiomyocytes and dopaminergic neurons as permissive to SARS-CoV-2 and shows that permissiveness does not track ACE2 expression alone.

2019 · Proceedings of the National Academy of Sciences · lab-led

Type I interferon response impairs differentiation potential of pluripotent stem cells

Forcing an interferon-stimulated gene program in human induced pluripotent stem cells with a constitutively active IRF7 produces lasting transcriptional change and impaired germ layer differentiation, supporting the proposal that the canonical type I interferon system and pluripotency are difficult to hold simultaneously.