tenOever LaboratoryVirology · Host defense · RNA biology
Technologies

Combinatorial peptide substrate specificity profiling

proteomics and biochemistry

Recorded terms: combinatorial peptide substrate specificity profiling

2022 · Science Signaling · collaborative

Host protein kinases required for SARS-CoV-2 nucleocapsid phosphorylation and viral replication

Kinase substrate specificity profiling assigns the phosphorylation cluster in the SARS-CoV-2 nucleocapsid SR-rich domain to a cascade initiated by SRPK1 and SRPK2 and extended by GSK-3 and casein kinase 1, whose inhibition suppresses coronavirus replication.