tenOever LaboratoryVirology · Host defense · RNA biology
Technologies

mSwAP-In iterative genome writing

genome engineering

Recorded terms: CRISPR-Cas9 assisted homologous recombination; mSwAP-In genome writing; yeast assembly of large DNA

2023 · Nature · collaborative

Mouse genome rewriting and tailoring of three important disease loci

An iterative, scarless and biallelic method for overwriting large mammalian genomic segments in mouse embryonic stem cells, used to build a recoded Trp53 locus and mice carrying the human ACE2 and TMPRSS2 loci in place of their mouse counterparts.