tenOever LaboratoryVirology · Host defense · RNA biology
Technologies

Phosphospecific immunoblotting and phosphatase treatment

proteomics and biochemistry

Recorded terms: phosphatase treatment; phosphospecific immunoblotting

2014 · Journal of Biological Chemistry · lab-led

Mitogen-activated Protein Kinase-mediated Licensing of Interferon Regulatory Factor 3/7 Reinforces the Cell Response to Virus

Sustained IRF7 activity induces the kinase MAP3K8, which phosphorylates the proline-rich hinge of IRF3 and redirects it from homodimers into IRF3 and IRF7 heterodimers, broadening the antiviral transcriptome and scaling the cellular response to the persistence of the viral threat.

2003 · Science · training period

Triggering the Interferon Antiviral Response Through an IKK-Related Pathway

The IKK-related kinases IKKepsilon and TBK1 are identified as components of the virus-activated kinase activity that phosphorylates the C-terminal serine cluster of IRF-3 and IRF-7, linking pathogen sensing to type I interferon gene induction and to establishment of an antiviral state.