tenOever LaboratoryVirology · Host defense · RNA biology
Technologies

Population diversity statistics and simulation

sequence and population analysis

Recorded terms: Monte Carlo simulation; Shannon diversity analysis

2020 · Journal of Virology · collaborative

Rapid Dissemination and Monopolization of Viral Populations in Mice Revealed Using a Panel of Barcoded Viruses

A library of 135 barcoded coxsackievirus B3 clones shows that orally inoculated virus reaches systemic tissues within 20 minutes and replicates as a diverse population, after which fewer than three variants come to dominate every tissue in the animal without any detectable adaptive mutation.

2014 · Cell Host & Microbe · lab-led

Influenza A Virus Transmission Bottlenecks Are Defined by Infection Route and Recipient Host

Genetically barcoded influenza A virus libraries tracked through cell culture, embryonated eggs, guinea pigs, ferrets and mice show that transmission bottlenecks differ by route and recipient, with airborne transmission reducing a diverse inoculum to as few as two founder clones.