RNA immunoprecipitation

small RNA methods

Recorded terms: RNA immunoprecipitation

2022 · Journal of Virology · collaborative

The Host Factor ANP32A Is Required for Influenza A Virus vRNA and cRNA Synthesis

Uncoupling the two steps of influenza A virus genome replication with viral promoter mutations shows that ANP32A is required for synthesis of both the complementary RNA intermediate and progeny genomic RNA, and that it acts on the actively replicating polymerase rather than the encapsidating one.

2012 · Journal of Virology · lab-led

A Small-RNA Enhancer of Viral Polymerase Activity

Influenza A virus small viral RNAs are shown to be synthesized from the complementary RNA intermediate, to load into the RNA binding cleft of the polymerase PA subunit, and to act there as segment-specific allosteric enhancers of full-length genome synthesis.