TechnologiesStem-loop quantitative RT-PCR for small RNAs
small RNA methods
Recorded terms: small RNA RT-qPCR; stem-loop quantitative RT-PCR
2017 · Cell Host & Microbe · collaborative
SARS-CoV generates three discrete small viral RNAs during infection of mouse lung, and blocking the one derived from the nucleocapsid gene with a locked nucleic acid antagomir reduces pulmonary inflammation, tissue damage and proinflammatory cytokine expression without measurably lowering lung virus titres.
2010 · Proceedings of the National Academy of Sciences · lab-led
Influenza A virus can be engineered to encode a cellular pri-microRNA inside an artificial intron of segment 8 and to produce mature, silencing-competent miR-124 during infection without measurable loss of replication or genome stability.